Question #4: We also will need 4 0.1 M NaCl in 0.05 M Tris-HCl (pH 7.5) buffer solution for the c...

Question #4: We also will need 4 0.1 M NaCl in 0.05 M Tris-HCl (pH 7.5) buffer solution for the column chromatography lab. YES, two chemicals in the same solution. Describe in detail how to prepare 2L of this buffer again using the buffer in question #2

Questions #2: Tris (or TRIZMA) is an abbreviation of the chemical: [Tris(hydroxymethyl)aminomethane] or 2-amino-2-(hydroxymethyl)-1,3-propanediol NH2 OH O HOH Tris has a molecular weight of 121.1 g/mol and when dissolved in H2O will result in a basic solution of pH 10.5